Cetyl-Pyridinium Chloride Is Useful for Isolation of Mycobacterium tuberculosis from Sputa Subjected to Long-Term Storage

Cetyl-pyridinium chloride is useful for isolation of Mycobacterium tuberculosis from sputa subjected to long-term storage.

Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 +/- 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subjected to long-term storage received from remote areas of the world.

Comparison of sodium carbonate, cetyl-pyridinium chloride, and sodium borate for preservation of sputa for culture of Mycobacterium tuberculosis.

Antimicrobial susceptibility testing of Mycobacterium tuberculosis is essential for a successful treatment of patients, mainly those with a previous history of antituberculosis therapy. Susceptibility testing of strains requires their isolation from sputum samples within 24 to 48 h of collection and storage at 2° to 4°C. If the sample is left at room temperature or in refrigeration for longer p...

Cetyl Pyridinium Chloride : I. Germicidal Properties.

Cetyl pyridinium chloride is a cationic detergent which is soluble in water, chloroform, alcohol, and acetone. Aqueous solutions are clear, colorless, and odorless. The agent has a depressant action upon surface tension. This property enhances the value of the germicide because irregular surfaces are uniformly wetted. The germicidal properties of cetyl pyridinium chloride were first studied in ...

Recovery rate of Mycobacterium tuberculosis from frozen AFB smear positive sputum 1 subjected to long term storage in Northwest Ethiopia

Rapid Detection of Mycobacterium tuberculosis in Sputa

A polymerase chain reaction (PCR) assay for the rapid detection of Mycobacterium tuberculosis in sputum samples was studied. The target DNAwas a 123-base pair (bp) fragment ofIS6110, which was repeated in the M.tuberculosis genomeand was specific for the M.tuberculosis complex. Glass beads (2mmdiameter) and lysozyme were used to lyse the mycobacteria and DNAwas extracted by the phenol-extractio...